Mycobacterium tuberculosis (Mtb) has a complex cell wall which contains multiple lipids presented by CD1 and recognized by T cells. αβ T cells specific for Mtb lipids are well studied, but γδ T cell responses are less characterized. In order to assess common TCR features of γδ CD1-lipid recognition, we stimulated peripheral blood mononuclear cells from healthy Mtb-exposed South African individuals and generated γδ T cell lines recognizing the mycobacterial lipids glucose monomycolate (GMM) and phosphomycoketide (PM). We index sorted over 300 antigen-specific T cells from these cell lines using CD1b-GMM and CD1c-PM tetramers and used plate-based single-cell RNA sequencing to define paired TCRγ and TCRδ chains. From one cell line we identified nine unique CD1c-PM-reactive γδ T cell clonotypes. We found that these γδ T cells exclusively express a Vδ1+ TCR and contain hydrophobic residues in the CDR3δ region, corroborating published findings and implying there may be conserved TCR patterns which contribute to CD1c-PM reactivity. We transiently transfected the two most frequent CD1c-PM-reactive γδ TCRs into HEK293T cells and confirmed their ability to bind CD1c-PM loaded tetramer, but not CD1c loaded with endogenous lipids. Analysis of the index sort data showed that tetramer avidity differed by TCR, and those differences were replicated in the transfected HEK293T cells. We also defined a Vδ1+ TCR reactive to CD1b-GMM, a specificity previously unreported in γδ T cells. This Vδ1 TCR also binds CD1b tetramers loaded with mycolic acid or endogenous lipids, consistent with a lipid-independent binding mechanism. Our future studies of CD1b-GMM and CD1c-PM γδ TCRs will test cross-reactivity with other lipids and aim to identify shared TCR features. A thorough study of these γδ T cells will answer questions about γδ T cell recognition patterns, help us understand potentially clinically relevant Mtb T cell responses, and inform future lipid-focused vaccination efforts.