Cell migration and function throughout the human body is a sophisticated process, orchestrated through a network of interactions between cells and receptors. This network is highly regulated to ensure the proper functioning of the immune system throughout life. Unconventional T cells play important and non-redundant roles in the human immune system. As unconventional T cells transition from the thymus to the blood of infants, and then subsequently mature in the periphery as we age, changes in frequency, phenotype and function are observed. Here, using spectral flow cytometry, we describe changes to NKT cells, MAIT cells and gamma delta (gd) T cells at birth compared to healthy adults aged 20-30 years and older adults aged 70-80 years. The proportion of MAIT cells and Vd2+ gd T cells were low in cord blood, and highest in adults aged 20-30 years, whereas Vd2+ gd T cells dropped in frequency in older adults and MAIT cells also trended lower. The frequency of NKT cells remained unchanged regardless of age, however, the phenotype of all three populations of unconventional T cells changes with age. Specifically, we found that chemokine receptors expressed by these subsets of unconventional T cells differed drastically throughout life. CCR4 and CCR7 significantly decreased in MAIT cells and Vd2+ gd T cells between cord blood and adult age groups. In contrast, Vd2- gd T cells had a lower proportion of CCR4 in cord blood compared to adults. Moreover, the expression of CCR6 significantly decreased only on Vd2+ gd T cells in older age groups compared to cord blood. Accordingly, our data highlights important changes in unconventional T cells as we age, which may have important implications for infection, cancer, and autoimmunity