The rising number of infection outbreaks is a major challenge for public health. The misuse of antibiotics and the lack of new drugs have added to the spread of drug-resistant bacteria. Mycobacterium tuberculosis (Mtb), the causative agent of Tuberculosis that devastated Europe during the first half of the 19th century, is still responsible for the death of over 1.6 million people annually, being the second deadliest infection after COVID-19. We have shown that polymeric nanoparticles containing isoniazid and clofazimine presented lack of toxicity, dose-dependent response, and improved therapeutic efficacy when compared to free drugs in vivo [1]. However, the site-specific delivery of drugs to infected cells remains elusive. Mucosal-associated invariant T (MAIT) cells exhibit the capacity to specifically recognise bacterial-derived metabolites (including from Mtb) presented by MHC class I-like related (MR1) protein. MR1 is an evolutionarily conserved protein that mainly resides in the endoplasmic reticulum of host cells but is readily translocated to the cell surface upon metabolite binding and association with β2 microglobulin (β2m). This translocation enables MR1 to function as a vigilant sensor for intracellular infection. Using phage display technology, we screened for peptides that bind specific MR1-metabolite complexes to directly target antibiotic-loaded polymersomes to infected cells. We have expressed and purified MR1 Ectodomain-β2m complexes with and without acetyl-6-formylpterin (Ac-6-FP), a synthetic analogue of 6-formylpterin (6-FP). 6-FP is an intermediate in folate biosynthesis pathways found in bacteria which has been shown to increase MR1 expression in the plasma membrane of cells in vitro. These purified complexes have been used as a target for phage display experiments. This study paves the way for the development of innovative approaches for targeted drug delivery through MR1-metabolite presentation, a system that is now known to be relevant not only in the context of bacterial infections but also in cancer and autoimmune disorders.