We recently described peptide-specific PLZF+ innate-like T (PILT) cells as a new member of the αβ innate-like T cells lineage in mice. Like other αβ innate-like T cells, PILT cells exhibit features of both adaptive and innate immunity. In particular, they acquire memory phenotype during development that allow them to respond quickly to cognate antigens and cytokine stimuli. Furthermore, and akin to iNKT and MAIT cells, PILT cell development was shown to be SLAMF signalling dependent and rely on double-positive thymocytes (DP):DP interaction during thymic selection. This suggests that PILT cells might share a similar transcriptional landscape as iNKT and MAIT cells. Yet unlike most αβ innate-like T cells which get selected by a non-peptide antigens presented on DP thymocytes, PILT cells get selected by DP thymocytes presenting peptides loaded on MHC I and/or MHC I-like molecules In this study, we compared PILT cells to iNKT cells on a transcriptional level using 10x genomics single-cell immune profiling (scRNA-seq) technology. Our analysis further validated the hypothesis that PILT and iNKT cells share a similar transcriptomic profile and follow comparable developmental trajectories in the thymus. Moreover, a subsequent single-cell V(D)J sequencing analysis showed that PILT cells utilise a variety of α and β TCR chains and display a polyclonal TCR repertoire, further emphasizing their diversity and adaptability.